Call of Juarez features classic gunslinging action including duels, shootouts and barroom brawls. Players will relive the days of the Wild West in numerous settings including local saloons and brothels.

In the single-player campaign, players enter the world of two opposing characters equipped with contrasting weapons and styles. Ray is the retired gunslinger turned rough-and-tumble preacher. Billy, a sneaky and elusive man befriended and trained by Native Americans, is on the run. Authentic Western situations chronicle the cat-and-mouse game played by Ray and Billy. Players are forced to make split-second decisions to escape the grasp of Ray or get their hands on Billy, and outwit secondary characters throughout this Western setting.